 |
|
| If you can't view the Datasheet, Please click here to try to view without PDF Reader . |
|
|
|
| If you can't view the Datasheet, Please click here to try to view without PDF Reader . |
|
|
| Datasheet File OCR Text: |
| Corporate Headquarters 400 Valley Road Warrington, PA 18976 1-800-523-2575 FAX 1-800-343-3291 Email: info@polysciences.com www.polysciences.com Europe - Germany Polysciences Europe GmbH Handelsstr. 3 D-69214 Eppelheim, Germany (49) 6221-765767 FAX (49) 6221-764620 Email: info@polysciences.de TECHNICAL DATA SHEET 741 Page 1 of 6 Microring Bacterial Detectors Polysciences offers a wide variety of products specific for microbiological and bacterial detection applications. Microring(R) bacterial detectors are simple, rapid test methods which utilize specific growth factors to identify different microorganisms. (R) Microring(R) XV Cat. #24644 (50 rings/pkg) Microring(R) XV is a paper ring used in Haemophilus identification. It comes complete with individual printed tips containing haemin (X-factor) and nicotinamide adenine dinucleotide-NAD (V-factor) or X+V factor. This ring differentiates Haemophilus species on the basis of growth requirements for X and V factors and can be identified by their growth patterns close to the tips. V-tip also contains -aminolaevulinic acid (ALA) for Porphyrin test. (The porphyrin test is best performed at the start when the ring is laid on the plate prior to incubation. Using a loop take a sample of the test organism from a growing culture and spread lightly over the V-tip. Non-haemin requiring species will cause an orange-red glow to be seen from the V-tip.) Procedure 1. Spread sample of the test organism onto an agar plate of Tryptone Soy Agar or Columbia Agar (without blood). 2. Using sterile forceps, carefully place Microring(R) XV in the center of the plate. Press each tip gently to ensure contact with surface. 3. Incubate plate overnight at 37 C. 4. Following incubation, growth patterns around each tip are read and used to identify the organism. Differentiation of Haemophilus species Species H. influenzae H. aegypticus H. parainfluenzae H. haemolyticus H. parahaemolyticus Growth results at tip XV V X NG NG G NG NG G G NG G NG NG NG G G G Growth factor requirement XV XV V XV V XV = Haemin and NAD Porphyrin synthesis (ALA) + + NG = No growth Haemolysis on horse blood* + + G = Zone of growth X = Haemin V = NAD and -aminolaevulinic acid (ALA) * Haemolysis recorded from primary culture. Microring(R) XV continues - pg 2 Should any of our materials fail to perform to our specifications, we will be pleased to provide replacements or return the purchase price. We solicit your inquiries concerning all needs for life sciences work. The information given in this bulletin is to the best of our knowledge accurate, but no warranty is expressed or implied. It is the user's responsibility to determine the suitability for their own use of the products described herein, and since conditions of use are beyond our control, we disclaim all liability with respect to the use of any material supplied by us. Nothing contained herein shall be construed as a recommendation to use any product or to practice any process in violation of any law or any government regulation. (c) Polysciences, Inc. Active: Jan/2007 Data Sheet #741 1 TECHNICAL DATA SHEET 741 Microring(R) XV Interpretation Growth factor requirements X V H. influenzae + + H. aegypticus + + H. parainfluenzae - + H. haemolyticus + + H. parahaemolyticus - + X = Haemin, V = NAD and -aminolaevulinic acid (ALA) Page 2 of 6 Synthesis of porphyrins from ala - - + - + Haenikysis on horse blood - - - + + Species Storage & Handling Store Microring(R) XV at 2-8C Rings maintain a shelf life of 24 months from manufacture date. Microring(R) AN Cat. #24645 (50 rings/pkg) Microring(R) AN is a filter paper ring used for the identification of Non-sporing Anaerobes. It has 6 tips which are impregnated with a range of antibiotics for the identification of species of anaerobic Gram-negative bacilli and can distinguish Gram-positive cocci from Gram-negative cocci. Gram-negative anaerobic rods are found in more than half of clinical specimens containing anaerobes. Although difficult to perform precise identifications, in practice most groups can be presumptively identified from their antibiotic susceptibility profile, which is the basis of the Microring(R) AN test scheme. Procedure 1. Spread sample of test organism onto a plate containing anaerobic agar. 2. Using sterile forceps, carefully place Microring(R) AN in the center of the plate. Press each tip gently to ensure contact with surface. 3. Incubate for 24-48 hours at 35-37 C under anaerobic conditions. 4. Following incubation, growth patterns around each tip are read and used to identify the organism. Zone diameters of 15mm or greater are considerd susceptible, while those less than 15mm are regarded as resistant. Code E RP CO PG K VA Color Red Dark red White Pink Salmon Blue Antibiotic Erythromycin Rifampicin Colistin Penicillin G Kanamycin Vancomycin Amount 60ug 15ug 10ug 2 units 1000ug 5ug Identification Table Species B. Fragilis Prevotella melaninogenicus Prevotella oralis B. ureolyticus E RP CO PG K VA S S S S S S S S R V S S R S S S R R R S R R R R Aesculin hydrolysis + - + - Growth in bile + - - - Indole + - - - Oxidase positive. Black pigmented colonies with brick red fluorescence. Other details E = Erythromycin, RP = Rifampicin, CO = Colistin, PG = Penicillin, K = Kanamycin, VA = Vancomycin S = Sensitive, R = Resistant Microring(R) AN continues - pg 3 Should any of our materials fail to perform to our specifications, we will be pleased to provide replacements or return the purchase price. We solicit your inquiries concerning all needs for life sciences work. The information given in this bulletin is to the best of our knowledge accurate, but no warranty is expressed or implied. It is the user's responsibility to determine the suitability for their own use of the products described herein, and since conditions of use are beyond our control, we disclaim all liability with respect to the use of any material supplied by us. Nothing contained herein shall be construed as a recommendation to use any product or to practice any process in violation of any law or any government regulation. (c) Polysciences, Inc. Active: Jan/2007 Data Sheet #741 1 TECHNICAL DATA SHEET 741 Microring(R) AN Identification Continued Species F. nucleatum F. varium F. mortiferum Gram-pos. cocci Gram-neg. cocci F. necrophorum E RP CO PG K VA S R R S S S S S S S S S S R R S R S S S S S S S S S S S S S R R R R S R - - + Lypolytic on egg yolk agar. S = Sensitive, R = Resistant Page 3 of 6 Aesculin hydrolysis - - + Growth in bile - + + Indole + + - Other details E = Erythromycin, RP = Rifampicin, CO = Colistin, PG = Penicillin, K = Kanamycin, VA = Vancomycin Microring(R) AN (GIFU METHOD) Recently a new identification scheme has been developed in Japan for use with Microring(R) AN. Prepare bacterial suspension as above, but adjust turbidity to between 1 and 3 MacFarland. This ensures adequate visible growth within 48hours. Using this inoculum, resistance and sensitivity are measured as follows: Size of Zone (mm) Interpretation < _10 Resistant (R) 11-14 Intermediate (I) > 15 _ Sensitive (S) Species B. fragilis Prevotella melaninogenicus Prevotella intermedia Other pigmented prevotella Prevotella oralis Non-pigmented Prevotella Porphyromonas B. ureolyticus F. nucleatum F. varium Gram-neg. cocci Campylobacter B. wadsworthia Desulfovibrio C. clostridioforme C. symbiosum Anaerbiospirillum D. pneumonsintes S. wadsorthensis L. buccalis C. ochracea E V S S S S S S S R R I/R S R S V S/I R I S R S RP S S S S S S S I S R S R R R R S S I S S S CO R V S S S S R S V S S S S R R R S R S S R PG R S V S S R S I S S R R R R R R R R R R I K R R R R R R R S S S V S S S S S S S S S R VA R R R R R R V R R R R R R R S S R R R R R E = Erythromycin RP = Rifampicin CO = Colistin PG = Penicillin K = Kanamycin VA = Vancomycin S = Sensitive R = Resistant V = Variable Storage & Handling Store Microring(R) AN at 2-8C Rings maintain a shelf life of 12 months from manufacture date. Should any of our materials fail to perform to our specifications, we will be pleased to provide replacements or return the purchase price. We solicit your inquiries concerning all needs for life sciences work. The information given in this bulletin is to the best of our knowledge accurate, but no warranty is expressed or implied. It is the user's responsibility to determine the suitability for their own use of the products described herein, and since conditions of use are beyond our control, we disclaim all liability with respect to the use of any material supplied by us. Nothing contained herein shall be construed as a recommendation to use any product or to practice any process in violation of any law or any government regulation. (c) Polysciences, Inc. Active: Jan/2007 Data Sheet #741 1 TECHNICAL DATA SHEET 741 Microring AC Cat. #24654 (50 rings/pkg) Microring(R) AC is a filter paper ring used for the identification of Gram-positive anaerobic cocci and some microaerophilic cocci. It has 3 individual printed tips containing various antimicrobial reagents. The resistance or sensitivity patterns can be used to help identify anaerobic cocci. In particular, P. anaerobius can be readily picked out by it's sensitivity to SPS and P. magnus can be distinguished from P. micros by its resistance to Novobiocin. (R) Page 4 of 6 Procedure 1. Spread sample of test organism onto a plate containing anaerobic agar. 2. Using sterile forceps, carefully place Microring(R) AC in the center of the plate. Press each tip gently to ensure contact with surface. 3. Incubate overnight at 37 C under anaerobic conditions. 4. Following incubation, growth patterns around each tip are read and used to identify the organism. Code MZ SPS NO Color White Pink Blue Antibiotic Metronidazole Sodium Polyanethol Sulphonate Novobiocin Amount 5ug 1mg 5ug Microring(R) AC Identification Species Peptococcus niger Peptostreptococcus asaccharolyticus Peptostreptococcus indolicus Peptostreptococcus magnus Anaerococcus prevotil Peptostreptococcus anaerobius Peptostreptococcus micros Peptostreptococcus productus Peptostreptococcus tetradius Atopbium parvulum Staphylococcus saccharolyticus Other microaerophilic streptococci Streptococcus intermedius Gemella morbillorum Streptococcus constellatus R R R S S S R R R L,a L,a L,a Metronidazole S S S S S S S S S S R/S S R Novobiocin R R R R R S S S SPS R R R R R S R R R R R Indole + + Fatty Acids from PYG broth* B,C,iv,a B,(A,p,l) B,(A,p,l) A B,A,(L),p IC,A,(iv,ib,b) A(s) A,l,s L,B(A,p) L,a F/A S = Suseptibility, R = Resistant, R/S = aerotolerant strains resistant (a) Occasional strains may give a zone size >12mm, but these organisms are easily distinguishable from Peptostreptococcus anaerobius as they are resistant to Novobicin. (b) Occasional strains may give a small zone to SPS < 12mm in diameter. _ * Gas-liquid chromotography analysis of fatty acid products in Peptone Yeast extract Glucose (PYG) broth. Capital letters indicate major metabolic products, small letters indicate minor products and parentheses indicate variable reaction. Storage & Handling Store Microring(R) AN at 2-8C Rings maintain a shelf life of 12 months from manufacture date. Should any of our materials fail to perform to our specifications, we will be pleased to provide replacements or return the purchase price. We solicit your inquiries concerning all needs for life sciences work. The information given in this bulletin is to the best of our knowledge accurate, but no warranty is expressed or implied. It is the user's responsibility to determine the suitability for their own use of the products described herein, and since conditions of use are beyond our control, we disclaim all liability with respect to the use of any material supplied by us. Nothing contained herein shall be construed as a recommendation to use any product or to practice any process in violation of any law or any government regulation. (c) Polysciences, Inc. Active: Jan/2007 Data Sheet #741 1 TECHNICAL DATA SHEET 741 Page 5 of 6 Microring(R) GV Cat. #24655 (50 rings/pkg) Microring(R) GV is a filter paper ring used for identification of Gardnerella vaginalis. The ring comes complete with 4 individual printed tips containing various antimicrobial reagents. Each tip of the ring contains one of the following reagents; MTZ (metronidazole), TRM (trimethoprim), STZ (sulphathiazole), and BIL, (bile salt). Each tip performs as a single identification disc because of an isolating hydrophobic barrier. On blood agar, Gardnerella is identified by sensitivity to 3 designated tips and resistance to 1 tip. Procedure 1. Spread sample of test organism onto a plate containing Columbia Agar with blood. 2. Using sterile forceps, carefully place Microring(R) GV in the center of the plate. Press each tip gently to ensure contact with surface. 3. Incubate plate for 48 hours at 37 C in humid atmosphere containing 5% CO2. 4. Following incubation, growth patterns around each tip are read and used to identify the organism. Species Gardnerella Vaginalis Vaginal Lactobacilli Cat -ve Corynebacter Mtz S R R Trm S R R Stz R V V Bil S R R Bile 5mg Metronidazole 50 mcg MTZ = metronidazole, TRM = trimethoprim, STZ = sulphathiazole, BIL = bile salt S = Susceptible, R = Resistant, V = Variable Storage & Handling Store at 4 C, avoid prolonged exposure to direct sunlight. Microring(R) GV should be handled with forceps. Avoid contact with skin to prevent contamination. Rings maintain a shelf life 12 of months from manufacture date. Sulphathiazole 1mg Trimethoprim 5 mcg Should any of our materials fail to perform to our specifications, we will be pleased to provide replacements or return the purchase price. We solicit your inquiries concerning all needs for life sciences work. The information given in this bulletin is to the best of our knowledge accurate, but no warranty is expressed or implied. It is the user's responsibility to determine the suitability for their own use of the products described herein, and since conditions of use are beyond our control, we disclaim all liability with respect to the use of any material supplied by us. Nothing contained herein shall be construed as a recommendation to use any product or to practice any process in violation of any law or any government regulation. (c) Polysciences, Inc. Active: Jan/2007 Data Sheet #741 1 TECHNICAL DATA SHEET 741 Microring(R) YT Cat. #24646 (50 rings/pkg) Microring(R) YT is a filter paper ring used for the identification of clinically important yeasts, especially Candida species. Microring(R) YT uses the organism's profile of susceptibilities to a small range of reagents as the basis of identification. Microring(R) YT carries 6 tips impregnated with a range of dyes and antifungal reagents. Each tip acts like an individual disc because of the isolating hydrophobic barrier. Following overnight incubation, the resistance/sensitivity results of the individual tips are used to produce a 6-figure code which is matched against a database to give the identity of the yeast. Page 6 of 6 Procedure 1. Spread sample of test organism onto a plate containing anaerobic agar. 2. Using sterile forceps, carefully place Microring(R) YT in the center of the plate. Press each tip gently to ensure contact with surface. 3. Incubate for 24-48 hours at 37 C under anaerobic conditions. 4. Following incubation, growth patterns around each tip are read and used to identify the organism. Tip # 1 2 3 4 5 6 Contents Janus green Ethidium Bromide Triphenol tetrazolium chloride (TTC) Brilliant green Cycloheximide Rhodamine 6G Color Code Purple Salmon White Turquoise White Fuschia Concentration 156ug 37ug 150ug 200ug 45ug 60ug Storage & Handling Store Microring(R) YT at 2-8C Rings maintain a shelf life of 12 months from manufacture date. Ordering Information Cat # 24644 24645 24654 24655 24646 Description Microring(R) XV Microring(R) AN Microring AC (R) Size 1pkg (50 rings) 1pkg (50 rings) 1pkg (50 rings) 1pkg (50 rings) 1pkg (50 rings) To Order: In The U.S. Call: 1-800-523-2575 * 215-343-6484 In The U.S. Fax: 1-800-343-3291 * 215-343-0214 In Germany Call: (49) 6221-765767 In Germany Fax: (49) 6221-764620 Order online anytime at www.polysciences.com Microring(R) GV Microring(R) YT Should any of our materials fail to perform to our specifications, we will be pleased to provide replacements or return the purchase price. We solicit your inquiries concerning all needs for life sciences work. The information given in this bulletin is to the best of our knowledge accurate, but no warranty is expressed or implied. It is the user's responsibility to determine the suitability for their own use of the products described herein, and since conditions of use are beyond our control, we disclaim all liability with respect to the use of any material supplied by us. Nothing contained herein shall be construed as a recommendation to use any product or to practice any process in violation of any law or any government regulation. (c) Polysciences, Inc. Active: Jan/2007 Data Sheet #741 1 |
Price & Availability of PTX1400
 |
|
|
|
|
All Rights Reserved © IC-ON-LINE 2003 - 2022 |
| [Add Bookmark] [Contact Us] [Link exchange] [Privacy policy] |
|
Mirror Sites : [www.datasheet.hk]
[www.maxim4u.com] [www.ic-on-line.cn]
[www.ic-on-line.com] [www.ic-on-line.net]
[www.alldatasheet.com.cn]
[www.gdcy.com]
[www.gdcy.net] |